LC-MS Method Validation
| 52 academic hours (52 hours independent learning), 2 ECTS |
| Target group | All who have preliminary knowledge about analytical chemistry, liquid chromatograpy and mass spectrometry. | ||||||||||||||||||
| Learning outcomes | The participant who has successfully passed the course knows: * the main performance parameters of analytical methods, what they show and which of them are particularly important in different situations; * the main mathematical concepts and tools in method validation; * the main approaches for evaluation of the performance parameters in the case of LC/MS analysis. The participant who has successfully passed the course is able to: * decide what data are needed for evaluating the different method performance parameters, understand the meaning of the available data and decide whether the available data are sufficient; * select the approach and design the experiments for obtaining suitable data; * quantify the relevant performance parameters using the available data and assess whether the obtained values are realistic; * assess the fitness of the method for the intended purpose based on the values of the evaluated performance parameters. | ||||||||||||||||||
| Subject matter | Topic | Volume in hours | Lecturers | ||||||||||||||||
| Practice-oriented on-line course on validation of analytical methods, specifically using LC-MS as technique. The course covered the following topics: • The concept, workflow and scope of validation; • Selectivity and identity confirmation, both via LC and via MS; • Linearity of signal, linear range, sensitivity and their relation to calibration; • Precision, trueness, accuracy, analyte stability and their interrelations; • Limit of detection and limit of quantitation; • Ruggedness and robustness. | 52 | Ivo Leito, Koit Herodes, Karin Kipper, Riin Rebane, Anneli Kruve, Hanno Evard, Maarja-Liisa Oldekop | |||||||||||||||||
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